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National Stock Number:
6515-01-566-9056
Federal Supply Class:
6515
National Item Identification Number:
015669056
Description:
TNF-ALPHA ELISA KIT
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Manufacturer Information:
| ET2010-1 | 4ZTH3 | ASSAYPRO LLC |
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Techincal Specification:
| PART NAME ASSIGNED BY CONTROLLING AGENCY | ASSYMAX HUMAN TUMOR NECROSIS FACTOR ALPHA (TNF-ALPHA) ELISA KIT | | SPECIAL FEATURES | THE ASSAYMAX HUMAN TNF-ALPHA ELISA KIT IS DESIGNED FOR DETECTION OF TNF-ALPHA IN HUMAN PLAMSA, SERUM OR CELL CULTURE SUPERNATANTS. THIS ASSAY EMPLOYS A QUANTITATIVE SANDWICH ENZYME IMMUNOASSAY TECHNIQUE THAT MEASURES TNF-ALPHA IN 4.5 HOURS. A MURINE MONOCLONAL ANTIBODYSPECIFIC FOR HUMAN TNF-ALPHA HAS BEEN PRE-COATED ONTO A MICROPLATE. TNF-ALPHA IN STANDARDS AND SAMPLES IS SANDWICHED BY THE IMMOBILIZED ANTIBODY AND A BIOTINYLATED POLYCLONAL ANTIBODY SPECIFIC FOR HUMAN TNF-ALPHA, WHICH IS RECOGNIZED BY A STREPTAVIDIN-PEROXIDASE CONJUGATE. ALL UNBOUND MATERAL IS THEN WASHED AWAY AND A PEROXIDASE ENZYME SUBSTRATE IS ADDED. THE COLOR DEVELOPMENT IS STOPPED AND THE INTENSITY OF THE COLOR IS MEASURED. |
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