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National Stock Number:
6515-01-566-9055
Federal Supply Class:
6515
National Item Identification Number:
015669055
Description:
PAI-I ELISA KIT
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Manufacturer Information:
| EP1100-1 | 4ZTH3 | ASSAYPRO LLC |
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Techincal Specification:
| PART NAME ASSIGNED BY CONTROLLING AGENCY | ASSAYMAX HUMAN PLASMINOGEN ACTIVATOR INHIBITOR-1 (PAI-1) ELISA KIT | | SPECIAL FEATURES | THE ASSAYMAX HUMAN PAI-1 ELISA KIT IS DESIGNED FOR DETECTION OF PAI-1 IN HUMAN PLASMA, TISSUE EXTRACTS OR CELL CULTURE SUPERNATANTS. THIS ASSAY EMPLOYS A QUANTITATIVE SANDWICH ENZYME IMMUNOASSAY TECHNIQUE THAT MEASURE PAI-1 IN 4 HOURS. A POLYCLONAL ANTIBODY SPECIFIC FOR PAI-1 HAS BEEN PRE-COATED ONTO A MICROPLATE. PAI-1 IN STANDARS AND SAMPLES IS SANDWICHED BY THE IMMOBILIZED ANTIBODY AND A BIOTINYLATED POLYCLONAL ANTIBODY SPECIFIC FOR PAI-1, WHICH IS RECOGNIZED BY A STREPTAVIDIN-PEROXIDASE CONJUGATE. ALL UNBOUND MATERIAL IS THEN WASHED AWAY AND A PEROXIDASE ENZYME SUBSTRATE IS ADDED. THE COLOR DEVELOPMENT IS STOPPED AND THE INTENSITY OF THE COLOR IS MEASURED. |
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